Search results for " sequence"

showing 10 items of 3643 documents

The Greenland shark Somniosus microcephalus—Hemoglobins and ligand-binding properties

2017

A large amount of data is currently available on the adaptive mechanisms of polar bony fish hemoglobins, but structural information on those of cartilaginous species is scarce. This study presents the first characterisation of the hemoglobin system of one of the longest-living vertebrate species (392 +/- 120 years), the Arctic shark Somniosus microcephalus. Three major hemoglobins are found in its red blood cells and are made of two copies of the same a globin combined with two copies of three very similar beta subunits. The three hemoglobins show very similar oxygenation and carbonylation properties, which are unaffected by urea, a very important compound in marine elasmobranch physiology.…

---0301 basic medicinegenetic structuresProtein ConformationGreenlandlcsh:MedicineRESONANCE RAMAN-SPECTRAHETERODONTUS-PORTUSJACKSONISpectrum Analysis RamanBiochemistrychemistry.chemical_compoundHemoglobinsProtein structureAMINO-ACID SEQUENCEAnimal CellsSequence Analysis ProteinRed Blood CellsUreaNOTOTHENIOID FISHESPost-Translational Modificationlcsh:ScienceHemeChondrichthyesMultidisciplinarybiologyChemistryOrganic CompoundsChemical ReactionsVertebrateEukaryotaMOLECULAR ADAPTATIONSMicrocephalusGlobinsChemistryBiochemistryOptical EquipmentVertebratesPhysical SciencesEngineering and TechnologyCellular TypesResearch ArticleEnvironmental MonitoringProtein BindingQUATERNARY STRUCTURESAllosteric regulationEquipmentSTRETCHING FREQUENCIESHeme03 medical and health sciencesOXYGEN-BINDINGbiology.animalAnimals14. Life underwaterGlobinHemoglobinPhotolysisBlood Cells030102 biochemistry & molecular biologyLaserslcsh:ROrganic ChemistryOrganismsChemical CompoundsBiology and Life SciencesProteinsxxxCell Biologybiology.organism_classificationCARTILAGINOUS FISHOxygen030104 developmental biologySomniosusFishSharkslcsh:QHemoglobinProtein MultimerizationELASMOBRANCH HEMOGLOBINElasmobranchiiPLoS ONE
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The uniform convergence of a double sequence of functions at a point and Korovkin-type approximation theorems

2020

Abstract In this paper, we introduce an interesting kind of convergence for a double sequence called the uniform convergence at a point. We give an example and demonstrate a Korovkin-type approximation theorem for a double sequence of functions using the uniform convergence at a point. Then we show that our result is stronger than the Korovkin theorem given by Volkov and present several graphs. Finally, in the last section, we compute the rate of convergence.

010101 applied mathematicsPure mathematicsGeneral MathematicsUniform convergence010102 general mathematicsPoint (geometry)0101 mathematicsType (model theory)01 natural sciencesDouble sequenceMathematicsGeorgian Mathematical Journal
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Sequential Nucleophilic−Electrophilic Reactions Selectively Produce Isomerically Pure Nona‐ B ‐Substituted o ‐Carborane Derivatives

2003

Nine equal substituents on the intensively studied o-carborane have been obtained for the first time by a combined nucleophilic-electrophilic reaction sequence. Iodine and methyl groups have been introduced to prove the generality of the method. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2003)

010405 organic chemistryChemistrychemistry.chemical_element010402 general chemistryIodine01 natural sciences0104 chemical sciences3. Good healthInorganic ChemistryReaction sequenceNucleophileElectrophileCarboraneOrganic chemistryBoronEuropean Journal of Inorganic Chemistry
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A Global Sensitivity Analysis Toolbox to Quantify Drivers of Vegetation Radiative Transfer Models

2017

Abstract Global sensitivity analysis (GSA) enables to gain insight into the functioning of radiative transfer models (RTMs) by identifying the driving input variables of RTM spectral outputs such as reflectance, fluorescence, or radiance. This contribution introduces automated radiative transfer models operator's (ARTMO’s) new GSA toolbox. With the GSA toolbox the majority of ARTMO’s available RTMs can be decomposed into its driving variables. For a selected RTM output, a GSA identifies the most influential and noninfluential input variables according to Sobol' first-order and total-order indices. The toolbox can process RTM spectral outputs for any kind of optical sensor setting within the…

010504 meteorology & atmospheric sciences0211 other engineering and technologiesProcess (computing)Sobol sequence02 engineering and technology01 natural sciencesToolboxOperator (computer programming)GeographyRadiative transferRadianceRange (statistics)Sensitivity (control systems)021101 geological & geomatics engineering0105 earth and related environmental sciencesRemote sensing
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High Resolution X-ray Spectroscopy of T Tauri Stars in the Taurus-Auriga Complex

2006

Differences have been reported between the X-ray emission of accreting and non-accreting stars. Some observations have suggested that accretion shocks could be responsible for part of the X-ray emission in Classical T Tauri stars (CTTS). We present high-resolution X-ray spectroscopy of nine pre-main sequence stars in order to test the proposed spectroscopic differences between accreting and non-accreting pre-main sequence stars. We use X-ray spectroscopy from the XMM-Newton Reflection Grating Spectrometers and the EPIC instruments. We interpret the spectra using optically thin thermal models with variable abundances, together with an absorption column density. For BP Tau and AB Aur we deriv…

010504 meteorology & atmospheric sciencesAstrophysics::High Energy Astrophysical PhenomenaFOS: Physical sciencesAstrophysicsAstrophysics::Cosmology and Extragalactic AstrophysicsAstrophysics01 natural sciencesSpectral line0103 physical sciencesAstrophysics::Solar and Stellar Astrophysics010303 astronomy & astrophysicsAstrophysics::Galaxy Astrophysics0105 earth and related environmental sciencesPhysicsAURIGAStar formationAstrophysics (astro-ph)Astronomy and AstrophysicsAbundance of the chemical elementsAccretion (astrophysics)StarsT Tauri star13. Climate actionSpace and Planetary ScienceAstrophysics::Earth and Planetary AstrophysicsMain sequence
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Some sedimentological and geochemical characters of the late Triassic Noto formation, source rock in the Ragusa basin (Sicily)

1990

URA 724 du CNRS a intégré UMR 6113 - ISTO CNRS Université d'Orléans; In the Ragusa basin (southeastern Sicily), the late Triassic Noto formation is considered as the main oil source rock. We provide a detailed description of the sedimentary facies determined from core samples, and discuss geochemical results, obtained for both kerogens and chloroform extractable hydrocarbons from samples where sedimentary organic matter is immature. Two main sedimentary sequences were encountered: (i) layers of limestones and of marls (or shales) alternating at a metric scale, and (ii) laminites having various types and carbonate contents. The high petroleum potentials (S2 up to 100 kg HC/t rock) are relate…

010504 meteorology & atmospheric sciencesGeochemistryMineralogy010502 geochemistry & geophysics01 natural sciencesSedimentary depositional environmentchemistry.chemical_compoundNotoGeochemistry and Petrology[SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/GeochemistryKerogenSedimentary organic matterParent rockSicily0105 earth and related environmental sciencesMaturity (geology)kerogen typeclay/carbonate sequencesRagusa basinTriassicSource rockchemistryFaciesSedimentary rocksource rock sedimentologymodeling of oil formationGeology
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Recombinant laccase from Pediococcus acidilactici CECT 5930 with ability to degrade tyramine

2017

Biogenic amines degradation by bacterial laccases is little known, so we have cloned and heterologously expressed, in E. coli, a new laccase from Pediococcus acidilactici CECT 5930 (Lpa5930), a lactic acid bacterium commonly found in foods able to degrade tyramine. The recombinant enzyme has been characterized by physical and biochemical assays. Here we report the optimization of expression and purification procedures of this laccase. DNA encoding sequence of laccase from P. acidilactici was amplified by PCR and cloned into the expression plasmid pET28a for induction by isopropyl-β-D-thiogalactoipyranoside. Protein expression was performed in E. coli BL21(DE3) harboring pGro7 plasmid expres…

0106 biological sciences0301 basic medicineArabinoseMolecular biologylcsh:MedicineLaccasesBiochemistryBiotecnologia01 natural sciencesSubstrate Specificitylaw.inventionDatabase and Informatics Methodschemistry.chemical_compoundlawRecombinant Protein PurificationCloning MolecularAmineslcsh:Sciencechemistry.chemical_classificationMultidisciplinaryABTSbiologyOrganic CompoundsTemperatureHydrogen-Ion ConcentrationTyramineRecombinant ProteinsEnzymesChemistryRecombination-Based AssayBiochemistryPhysical SciencesRecombinant DNAElectrophoresis Polyacrylamide GelOxidation-ReductionSequence AnalysisResearch ArticleProtein PurificationBioinformaticsTyramineLibrary ScreeningDNA constructionResearch and Analysis Methods03 medical and health sciencesBacterial ProteinsSequence Motif Analysis010608 biotechnologyAmino Acid SequenceBenzothiazolesPediococcus acidilacticiLaccaseMolecular Biology Assays and Analysis TechniquesBase SequenceMolecular massLaccaseOrganic Chemistrylcsh:RChemical CompoundsBiology and Life SciencesProteinsPediococcus acidilacticiSequence Analysis DNAbiology.organism_classificationMolecular biology techniques030104 developmental biologyEnzymechemistryPlasmid ConstructionEnzymologySpectrophotometry Ultravioletlcsh:QSulfonic AcidsEnzimsProteïnesPurification TechniquesPLOS ONE
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Host dispersal shapes the population structure of a tick-borne bacterial pathogen

2020

Birds are hosts for several zoonotic pathogens. Because of their high mobility, especially of longdistance migrants, birds can disperse these pathogens, affecting their distribution and phylogeography. We focused on Borrelia burgdorferi sensu lato, which includes the causative agents of Lyme borreliosis, as an example for tick-borne pathogens, to address the role of birds as propagation hosts of zoonotic agents at a large geographical scale. We collected ticks from passerine birds in 11 European countries. B. burgdorferi s.l. prevalence in Ixodes spp. was 37% and increased with latitude. The fieldfare Turdus pilaris and the blackbird T. merula carried ticks with the highest Borrelia prevale…

0106 biological sciences0301 basic medicineBORRELIA-BURGDORFERIACARImedicine.disease_causemigrationBURGDORFERI SENSU-LATO01 natural sciencesSongbirdsLyme diseaseTicksAcariMigration11832 Microbiology and virologyLyme DiseasebiologyPlan_S-Compliant_NOBLACKBIRDS TURDUS-MERULAPREVALENCEEuropehost-parasite interactionsMIGRATORY BIRDSinternational1181 Ecology evolutionary biology[SDE]Environmental SciencesHost-paraste InterationsHost-parasite interactionsAvesTRANSMISSIONZoology010603 evolutionary biologyticksBirds03 medical and health sciencesBorreliaparasitic diseasesGeneticsmedicineAnimalsHumansBorrelia burgdorferiEcology Evolution Behavior and SystematicsLyme borreliosisInfecções Sistémicas e ZoonosesIxodesBird DiseasesBorreliaLYME-DISEASEIXODES-RICINUS TICKSBorrelia gariniiEcologíabiology.organism_classificationmedicine.diseasebacterial infections and mycoses030104 developmental biologybirdsCandidatusWILD BIRDSMultilocus sequence typingBorrelia gariniiIxodesMultilocus Sequence Typing
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A Metabarcoding Survey on the Fungal Microbiota Associated to the Olive Fruit Fly.

2017

The occurrence of interaction between insects and fungi is interesting from an ecological point of view, particularly when these interactions involve insect pests and plant pathogens within an agroecosystem. In this study, we aimed to perform an accurate analysis on the fungal microbiota associated to Bactrocera oleae (Rossi) through a metabarcoding approach based on 454 pyrosequencing. From this analysis, we retrieved 43,549 reads that clustered into 128 operational taxonomic units (OTUs), of which 29 resulted in the “core” associate fungi of B. oleae. This fungal community was mainly represented by sooty mould fungi, such as Cladosporium spp., Alternaria spp. and Aureobasidium spp., by pl…

0106 biological sciences0301 basic medicineBactrocera oleaeOlive fruit flySoil ScienceAureobasidium01 natural sciences03 medical and health sciencesMicrobial ecologyPseudocercospora454 PyrosequencingAscomycotaOleaBotanyColletotrichumAnimalsDNA Barcoding TaxonomicPseudocercosporaEcology Evolution Behavior and SystematicsEcological nicheHigh-throughput sequencingEcologybiologyBase SequenceEcologyfungiTephritidaefood and beveragesSettore AGR/12 - Patologia VegetaleAlternariaHigh-Throughput Nucleotide SequencingSequence Analysis DNAbiology.organism_classificationAlternaria030104 developmental biologySettore AGR/11 - Entomologia Generale E ApplicataColletotrichumDNA Intergenic454 Pyrosequencing; Bactrocera oleae; Cladosporium; Colletotrichum; High-throughput sequencing; PseudocercosporaCladosporium010606 plant biology & botanyCladosporiumMycobiomeMicrobial ecology
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A non-venomous sPLA2 of a lepidopteran insect: Its physiological functions in development and immunity

2018

Eicosanoids are oxygenated C20 polyunsaturated fatty acids that mediate various physiological processes in insects. Eicosanoid biosynthesis begins with a C20 precursor, arachidonic acid (5,8,11,14-eicosatetraenoic acid: AA). AA is usually released from phospholipids at sn-2 position by catalytic activity of phospholipase A2 (PLA2). Although various PLA2s classified into 16 gene families (= Groups) are known in various biological systems, few PLA2s are known in insects. Only two PLA2s involved in intracellular calcium independent PLA2 (iPLA2) group have been identified in lepidopteran insects with well known eicosanoid physiology. This study reports the first secretory PLA2 (sPLA2) in lepido…

0106 biological sciences0301 basic medicineCellular immunitybiologyfungiImmunologySpodopterabiology.organism_classification01 natural sciences010602 entomology03 medical and health sciencesOpen reading framechemistry.chemical_compound030104 developmental biologyPhospholipase A2BiochemistrychemistryJuvenile hormoneExiguabiology.proteinlipids (amino acids peptides and proteins)Arachidonic acidPeptide sequenceDevelopmental BiologyDevelopmental & Comparative Immunology
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